UTILIZAÇÃO DO kDNA PCR NO DIAGNÓSTICO MOLECULAR DE LEISHMANIOSES / USING kDNA PCR FOR THE MOLECULAR DIAGNOSIS OF LEISHMANIASIS
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چکیده
منابع مشابه
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL
This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the tradit...
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It is important to develop new methods for diagnosing relapses in the co-infection of visceral leishmaniasis (VL) and HIV to enable earlier detection using less invasive methods. We report a case of a co-infected patient who had relapses after VL treatment, where the qualitative kDNA PCR showed a good performance. The kDNA PCR seems to be a useful tool for diagnosing VL and may be a good marker...
متن کاملCould kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemio...
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BACKGROUND The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain segment of a conserved region of the mammalian gapdh gene. METHODOLOGY The proposed multiplex prot...
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Background: Arthritis is one of the most common inflammatory diseases worldwide. It is characterized by symptoms such as systemic inflammation and autoantibody production. The molecular mechanisms in pathogenesis of arthritis are not fully understood. Studies show that many microorganisms, including Mycoplasmas, play a role in arthritis. The PCR method is a fast and accurate molecular method fo...
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ژورنال
عنوان ژورنال: Ars Veterinaria
سال: 2013
ISSN: 2175-0106,0102-6380
DOI: 10.15361/2175-0106.2013v29n4p86